Thermo Fisher Scientific L1/ORF2 Polyclonal Antibody
L1/ORF2 단백질의 엔도뉴클레아제 도메인을 인식하는 닭 유래 폴리클로날 항체. Western blot과 ELISA에 사용 가능하며, 인간 및 침팬지 단백질에 반응. 연구용으로 L1 retrotransposon의 발현 및 기능 연구에 적합.
- 카탈로그번호
- 200-901-B20x (2개 옵션)
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific L1/ORF2 Polyclonal Antibody
Applications
Western Blot (WB)
- Tested Dilution: 1:2,000–1:10,000
ELISA
- Tested Dilution: 1:10,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Chimpanzee, Human |
| Host / Isotype | Chicken / IgY |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Prepared from eggs of chickens immunized with two synthetic peptides conjugated to KLH, corresponding to regions within the endonuclease domain of L1/ORF2 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Selective precipitation |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, Avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid repeated freeze/thaw cycles. Centrifuge product if not completely clear after standing at room temperature. Stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
L1/ORF2 Antibody is directed against two regions within the endonuclease domain of L1 ORF2 protein. Reactivity occurs against human L1/ORF2 protein and is useful in determining its presence in various assays. BLAST analysis suggests cross-reactivity with L1/ORF2 proteins from chimpanzee (100% homology) and from macaque, fruit fly, cattle, dog, opossum, and rat (69–88% homology).
Target Information
The L1 (LINE-1) or Long INterspersed Element retrotransposon ORF2 is a common open reading frame in the human genome, present in thousands of copies. This family includes magnesium-dependent endonucleases and phosphatases involved in intracellular signaling. Both ORF1 and ORF2 are required for autonomous retrotransposition. ORF2 encodes endonuclease, reverse transcriptase, and zinc knuckle domains. Its expression is tightly regulated except in germ line tissues, embryonic tissues, and certain cancers (teratomas, testicular cancers, leukemias). This antibody is intended for studying the control of L1 retrotransposons.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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