Thermo Fisher Scientific Phospho-EEF2 (Thr56) Recombinant Rabbit Monoclonal Antibody (17H31L21)
Phospho-EEF2 (Thr56)을 인식하는 재조합 토끼 단클론 항체로, Western blot 및 ChIP assay에 적합합니다. 높은 특이성과 민감도를 제공하며, 로트 간 일관성이 뛰어납니다. Human과 Mouse에 반응하며, 단백질 번역 과정 연구에 유용합니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 2.5 µg/mL | View 1 publication |
| ChIP assay (ChIP) | – | View 3 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Human, Pig |
| Host / Isotype | Rabbit / IgG |
| Expression System | Expi293 |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 17H31L21 |
| Immunogen | Peptide corresponding to human Phospho-EEF2 (Thr56) [aa54–aa61] |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C (short term). For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2723465 |
Product Specific Information
This antibody is predicted to react with Monkey, Pig, Cat, and Bovine.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The specific antibody DNA sequences from immunoreactive rabbits are cloned and expressed, then screened to select optimal candidates.
Advantages include:
- Improved specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broad immunoreactivity due to rabbit immune repertoire
Target Information
Phospho-EEF2 catalyzes the GTP-dependent ribosomal translocation step during translation elongation. In this step, the ribosome transitions from the pre-translocational (PRE) to the post-translocational (POST) state, moving the A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA to the P and E sites, respectively. This process coordinates tRNA movement, mRNA translocation, and ribosomal conformational changes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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