Thermo Fisher Scientific H3K9me1 Recombinant Rabbit Monoclonal Antibody (RM150), ChIP-Verified
Histone H3의 Lys9 단일 메틸화(K9me1)에 특이적으로 반응하는 재조합 토끼 단클론 항체입니다. ChIP, WB, ICC/IF, ELISA, Luminex 등 다양한 응용에 최적화되어 있으며, 교차 반응이 거의 없습니다. 단백질 A로 정제된 고순도 항체로, -20°C에서 안정적으로 보관 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.5–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 0.5–2 µg/mL |
| ELISA | 0.5–2 µg/mL |
| ChIP assay (ChIP) | 2–10 µg/mL |
| Luminex (LUM) | 0.1–0.5 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | RM150 |
| Immunogen | A monomethyl-peptide corresponding to Monomethyl-Histone H3 (Lys9) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS with 50% glycerol, 1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | -20°C |
| RRID | AB_2866512 |
Product Specific Information
This antibody specifically reacts with Histone H3 monomethylated at Lysine 9 (K9me1).
No cross-reactivity with non-modified Lysine 9, dimethylated Lysine 9 (K9me2), trimethylated Lysine 4 (K9me3), or other histone H3 methylations.
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Target Information
Histone H3 is a DNA-binding protein found in the chromatin of all eukaryotic cells. Together with other core histones, it forms nucleosomes by binding to DNA.
Histones play a central role in transcription regulation, DNA repair, replication, and chromosomal stability.
The N-terminal tail of histone H3 undergoes various post-translational modifications, such as methylation, acetylation, and phosphorylation, which influence chromatin structure and gene expression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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