Thermo Fisher Scientific H4K12ac Recombinant Rabbit Monoclonal Antibody (RM202), ChIP-Verified

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5-2 µg/mL

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Immunocytochemistry (ICC/IF)

0.5-2 µg/mL

-

ELISA (ELISA)

0.5-1 µg/mL

-

ChIP assay (ChIP)

1-5 µg

-

Luminex (LUM)

0.5-2 µg/mL

-

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

RM202

Immunogen

An acetyl-peptide corresponding to Acetyl-Histone H4 (Lys12) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:H4K12ac,uniProtId:P62805-1,ncbiNodeId:9606,antigenRange:12,antigenLength:103,antigenImageFileName:MA5-33388-20UG_H4K12ac_P62805-1_Rabbit.svg,antigenImageFileNamePDP:MA5-33388-20UG_H4K12ac_P62805-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS with 50% glycerol, 1% BSA

Contains

0.09% sodium azide

Storage conditions

-20°C

RRID

AB_2866515

Product Specific Information

This product reacts to Histone H4 acetylated at Lysine 12 (K12ac). No cross reactivity with other acetylated Lysines in Histone H4.

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Target Information

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.