Thermo Fisher Scientific H4K12ac Recombinant Rabbit Monoclonal Antibody (RM202), ChIP-Verified
Histone H4K12ac 특이적 recombinant rabbit monoclonal antibody로, ChIP 검증 완료. Western blot, ICC/IF, ELISA, ChIP assay, Luminex 등 다양한 응용에 사용 가능. Human 시료 반응, Protein A 정제, -20°C 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.5–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 0.5–2 µg/mL |
| ELISA | 0.5–1 µg/mL |
| ChIP assay (ChIP) | 1–5 µg |
| Luminex (LUM) | 0.5–2 µg/mL |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | RM202 |
| Immunogen | Acetyl-peptide corresponding to Acetyl-Histone H4 (Lys12) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS with 50% glycerol, 1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | -20°C |
| RRID | AB_2866515 |
Product Specific Information
This antibody reacts specifically to Histone H4 acetylated at Lysine 12 (K12ac) and shows no cross-reactivity with other acetylated lysines in Histone H4.
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Target Information
Histones are basic nuclear proteins responsible for the nucleosome structure of chromatin in eukaryotes. Two molecules each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units called nucleosomes. The linker histone H1 interacts with linker DNA between nucleosomes and aids in chromatin compaction into higher-order structures.
The gene encoding Histone H4 is intronless, lacks polyA tails, and contains a palindromic termination element. It is located within the large histone gene cluster on chromosome 6.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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