Thermo Fisher Scientific H3K9me1 Recombinant Rabbit Monoclonal Antibody (RM150), ChIP-Verified

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5-2 µg/mL

-

Immunocytochemistry (ICC/IF)

0.5-2 µg/mL

-

ELISA (ELISA)

0.5-2 µg/mL

-

ChIP assay (ChIP)

2-10 µg/mL

-

Luminex (LUM)

0.1-0.5 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

RM150

Immunogen

A monomethyl-peptide corresponding to Monomethyl-Histone H3 (Lys9) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:H3K9me1,uniProtId:P68431-1,ncbiNodeId:9606,antigenRange:9,antigenLength:136,antigenImageFileName:MA5-33385_H3K9me1_P68431-1_Rabbit.svg,antigenImageFileNamePDP:MA5-33385_H3K9me1_P68431-1_Rabbit_PDP.jpeg,sortOrder:1},{targetFamily:H3K9me1,uniProtId:Q71DI3-1,ncbiNodeId:9606,antigenRange:9,antigenLength:136,antigenImageFileName:MA5-33385_H3K9me1_Q71DI3-1_Rabbit.svg,antigenImageFileNamePDP:MA5-33385_H3K9me1_Q71DI3-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS with 50% glycerol, 1% BSA

Contains

0.09% sodium azide

Storage conditions

-20°C

RRID

AB_2815523

Product Specific Information

This product reacts to Histone H3 monomethylated at Lysine 9 (K9me1). No cross reactivity with non-modified Lysine 9, dimethylated Lysine 9 (K9me2), trimethylated Lysine 4 (K9me3), or other methylations in histone H3.

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Target Information

Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3 along with four core histone proteins binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Post translationally, histones are modified in a variety of ways to either directly change the chromatin structure or allow for the binding of specific transcription factors. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.