Thermo Fisher Scientific H3K9me1 Recombinant Rabbit Monoclonal Antibody (RM150), ChIP-Verified
Histone H3K9me1에 특이적인 recombinant rabbit monoclonal antibody로, Western blot, ICC/IF, ELISA, ChIP 등 다양한 응용에 적합합니다. 높은 특이성과 재현성을 제공하며, ChIP 검증 완료 제품입니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.5–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 0.5–2 µg/mL |
| ELISA | 0.5–2 µg/mL |
| ChIP assay (ChIP) | 2–10 µg/mL |
| Luminex (LUM) | 0.1–0.5 µg/mL |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | RM150 |
| Immunogen | A monomethyl-peptide corresponding to Monomethyl-Histone H3 (Lys9) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS with 50% glycerol, 1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | -20°C |
| RRID | AB_2815523 |
Product Specific Information
This antibody specifically recognizes Histone H3 monomethylated at Lysine 9 (H3K9me1).
No cross-reactivity with non-modified Lysine 9, dimethylated Lysine 9 (K9me2), trimethylated Lysine 4 (K9me3), or other histone H3 methylations.
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Target Information
Histone H3 is a DNA-binding protein present in the chromatin of all eukaryotic cells. Together with other core histones, it forms the nucleosome structure that organizes DNA.
Histone H3 plays a vital role in transcription regulation, DNA repair, replication, and chromosomal stability.
Its N-terminal tail undergoes multiple post-translational modifications, such as methylation, acetylation, and phosphorylation, influencing chromatin structure and gene expression.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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