Thermo Fisher Scientific VEGF Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Product Specific Information

• In Western blot, a band is observed at approximately 42 kDa with a chimera transfected lysate representing the homodimer VEGFA.
• A faint band at approximately 21 kDa represents the monomer form of VEGFA.
• This antibody is not recommended for IHC applications.
• Suggested positive control: antigen standard for VEGFA (transient overexpression lysate).

Target Information

VEGF (vascular endothelial growth factor) is a 45 kDa homodimeric, disulfide-linked glycoprotein involved in angiogenesis, promoting tumor progression and metastasis.
It affects vascular endothelium by promoting endothelial cell viability, mitogenesis, chemotaxis, and vascular permeability.

The VEGF family includes VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGF-E, and PIGF.
VEGF and its receptors (FLT1/VEGFR-1, FLK1/KDR/VEGFR-2) are key regulators in angiogenesis signaling and are primarily expressed on endothelial cells.
Alternative splicing of the VEGF gene produces five isoforms, with one form (sFLT1) acting as an inhibitor of angiogenesis.
Elevated VEGF levels are associated with POEMS syndrome (Polyneuropathy, Organomegaly, Endocrinopathy, Monoclonal gammopathy, Skin changes), also known as Crow-Fukase syndrome.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.