Thermo Fisher Scientific MCP-1 Polyclonal Antibody, PeproTech
Mouse MCP-1(CCL2)에 특이적인 Rabbit Polyclonal Antibody로, Western Blot, ELISA, IHC 등 다양한 실험에 사용 가능. 항원 친화 크로마토그래피로 정제되어 높은 특이성과 민감도 제공. 연구용으로만 사용 가능.
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Thermo Fisher Scientific MCP-1 Polyclonal Antibody, PeproTech
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL | View 2 publications |
| Immunohistochemistry (IHC) | – | View 1 publication |
| ELISA | 0.5–2.0 µg/mL | View 1 publication |
| Neutralization (Neu) | – | View 1 publication |
| In vitro Assay (IV) | – | View 1 publication |
| Immunostaining (IS) | – | View 2 publications |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host/Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Murine JE/MCP-1 (CCL2) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2929269 |
Product Specific Information
AA Sequence of recombinant protein:
QPDAVNAPLT CCYSFTSKMI PMSRLESYKR ITSSRCPKEA VVFVTKLKRE VCADPKKEWV QTYIKNLDRN QMRSEPTTLF KTASALRSSA PLNVKLTRKS EANASTTFST TTSSTSVGVT SVTVN
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Murine JE/MCP-1 (CCL2). Anti-Murine JE/MCP-1 (CCL2)-specific antibody was purified by affinity chromatography employing an immobilized Murine JE/MCP-1 (CCL2) matrix.
Sandwich ELISA:
To detect Murine JE/MCP-1 (CCL2) by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Murine JE/MCP-1 (CCL2) (500-P113Bt) as a detection antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant Murine JE/MCP-1 (CCL2).
Western Blot:
To detect Murine JE/MCP-1 (CCL2) by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used in conjunction with compatible secondary reagents, the detection limit for Recombinant Murine JE/MCP-1 (CCL2) is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are secreted proteins involved in immunoregulatory and inflammatory processes. The encoded protein is structurally related to the CXC subfamily of cytokines, characterized by two cysteines separated by a single amino acid.
This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It is implicated in diseases with monocytic infiltrates, such as psoriasis, rheumatoid arthritis, and atherosclerosis, and binds to chemokine receptors CCR2 and CCR4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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