Thermo Fisher Scientific MCP-1 Polyclonal Antibody, PeproTech

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-0.2 µg/mL

View 2 publications 2 publications

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

ELISA (ELISA)

0.5-2.0 µg/mL

View 1 publication 1 publication

Neutralization (Neu)

-

View 1 publication 1 publication

In vitro Assay (IV)

-

View 1 publication 1 publication

Immunostaining (IS)

-

View 2 publications 2 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rabbit

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived Recombinant Murine JE/MCP-1 (CCL2) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CCL2 (MCP-1),uniProtId:P10148-1,ncbiNodeId:10090,antigenRange:24-148,antigenLength:148,antigenImageFileName:500-P113-100UG_CCL2_MCP-1_P10148-1_Rabbit.svg,antigenImageFileNamePDP:500-P113-100UG_CCL2_MCP-1_P10148-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.1-1.0 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

RRID

AB_2929269

Product Specific Information

AA Sequence of recombinant protein: QPDAVNAPLT CCYSFTSKMI PMSRLESYKR ITSSRCPKEA VVFVTKLKRE VCADPKKEWV QTYIKNLDRN QMRSEPTTLF KTASALRSSA PLNVKLTRKS EANASTTFST TTSSTSVGVT SVTVN.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Murine JE/MCP-1 (CCL2). Anti-Murine JE/MCP-1 (CCL2)-specific antibody was purified by affinity chromatography employing an immobilized Murine JE/MCP-1 (CCL2) matrix.

Sandwich ELISA: To detect Murine JE/MCP-1 (CCL2) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Murine JE/MCP-1 (CCL2) (500-P113Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Murine JE/MCP-1(CCL2).

Western Blot: To detect Murine JE/MCP-1 (CCL2) by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Murine JE/MCP-1 (CCL2) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Target Information

This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.