Thermo Fisher Scientific IL-2 Polyclonal Antibody, Biotin, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
PTSSSTSSST AEAQQQQQQQ QQQQQHLEQL LMDLQELLSR MENYRNLKLP RMLTFKFYLP KQATELKDLQ CLEDELGPLR HVLDLTQSKS FQLEDAENFI SNIRVTVVKL KGSDNTFECQ FDDESATVVD FLRRWIAFCQ SIISTSPQ

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Murine IL-2. Anti-Murine IL-2-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect Murine IL-2 by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Murine IL-2 (500-P111) as a capture antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant Murine IL-2.

Western Blot:
To detect mIL-2 by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used with compatible secondary reagents, the detection limit for Recombinant mIL-2 is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.

Packaging:
500-P111BT-1MG will be provided as 2 × 500 µg.

Target Information

Interleukin 2 (IL-2) is an immuno-modulatory cytokine important for the proliferation of activated T cells, and differentiation of B cells, NK cells, monocytes, and macrophages. IL-2 signals through the IL-2 receptor (IL-2R), a heterotrimeric protein complex whose gamma chain is also shared by IL-4 and IL-7. Expression of the IL-2 gene in mature thymocytes is monoallelic, representing a unique regulatory mechanism. Disruption of the IL-2 gene in mice leads to an ulcerative colitis-like disease, highlighting its essential role in immune regulation.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.