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Takara E. coli chemically competent cells
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Takara E. coli chemically competent cells

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Takara의 E. coli 화학적 형질전환 세포는 다양한 클로닝, 돌연변이 유도, 대형 DNA 삽입 및 메틸화 DNA 클로닝에 최적화된 고효율 세포입니다. 여러 균주 선택 가능하며 안정적인 유전자형과 높은 형질전환 효율을 제공합니다. 연구용으로만 사용됩니다.

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Competent Cells
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Takara 9051 E. Coli HB-101 Competent Cells, 10 x 100 ul pk판매 단위 pk ·
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Takara 9053 E coli Competent Cells CJ236, 10 x 100 ul pk판매 단위 pk ·
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Takara 9057 E. coli DH5a Competent Cells, 10 x 100 ul pk판매 단위 pk ·
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Takara · Takara E. coli chemically competent cells

Takara E. coli Chemically Competent Cells

Applications

  • Recombinant DNA cloning experiments (library preparation, recombinant plasmid generation, subcloning)
  • Site-directed mutagenesis (HST02 and BMH71-18 mutS)
  • Cloning methylated DNA (HST08 or HST16CR)
  • Cloning large DNA fragments (HST08 or HST16CR)

Purity

No colonies form after inoculation of LB-agar medium containing 100 µg/ml ampicillin with 100 µl of E. coli competent cells.

Genotypes

Strain Genotype
E. coli HB101 (Cat. #9051) e14–(mcrA_–), _supE_44, _relA_1, Δ(_lac-proAB)/F` [traD_36, _proAB+, _lac I_q, _lacZ_ΔM15_]
E. coli CJ236 (Cat. #9053) dut_1, _ung_1, _thi-1, relA_1/pCJ105(F` _cam_r)
E. coli BMH71-18 mutS (Cat. #9054) Δ(lac-proAB), supE, thi-1, mutS_215::Tn_10(tet_r)/F` [traD_36, _proAB+, _lac I_q, _lacZ_ΔM15_]
E. coli MV1184 (Cat. #9055) ara, Δ(lac-proAB), rpsL, thi (ø80_lacZ_ΔM15), Δ(srl-recA)306::Tn_10_(tet_r)/F`[traD_36, _proAB+, _lac I_q, _lacZ_ΔM15_]
E. coli HST02 (Cat. #9127) F`[tra_D36, _pro_A+B+, _lac_Iq, _lac_ZΔM15_]/Δ(_lac-proAB), recA, endA, gyrA96, thi, e14–(mcrA–), supE44, relA, ΔdeoR, Δ(mrr-hsd_RMS-mcr_BC)
E. coli DH5α (Cat. #9057) F–, ø80d_lacZ_ΔM15, Δ(lacZYA-argF)U169, deoR, recA1, endA1, hsdR17(rK–, mK+), phoA, supE44, λ–, thi-1, gyrA96, relA1
E. coli HST08 (Cat. #9128) F–, endA1, supE44, thi-1, recA1, relA1, gyrA96, phoA, ø80d_lacZ_ΔM15, Δ(lacZYA-argF)U169, Δ(mrr-hsd_RMS-mcr_BC), ΔmcrA, λ–
E. coli HST16CR (Cat. #9131) F–, endA1, supE44, thi-1, recA1, relA1, gyrA96, phoA, ø80d_lacZ_ΔM15, Δ(lacZYA-argF)U169, Δ(mrr-hsd_RMS-mcr_BC), ΔmcrA, ΔpcnB, λ–

Uses of Each Strain

  • E. coli HB101: Stable genotype; used as a general-purpose strain for recombinant DNA experiments.
  • E. coli JM109: Used for alpha-complementation (blue/white screening) and ssDNA preparation; ideal for library construction and subcloning.
  • E. coli CJ236: Used for preparation of uracil-containing ssDNA (Kunkel’s method); cultivation requires chloramphenicol for F` episome maintenance.
  • E. coli BMH71-18 mutS: Used for site-directed mutagenesis; lacks mismatch repair capability, increasing mutagenesis efficiency.
  • E. coli MV1184: Used to select amber mutants; suitable as M13 phage or phagemid vector host for ssDNA preparation.
  • E. coli HST02: Restriction-minus phenotype allows efficient cloning of methylated DNA; suitable for alpha-complementation and library preparation.
  • E. coli DH5α: General-purpose cloning strain; economical blue/white screening without IPTG.
  • E. coli HST08: Lacks restriction genes (mrr, hsd_RMS, mcr_BC, mcrA); recommended for cloning large (>10 kb) or methylated DNA fragments.
  • E. coli HST16CR: Similar to HST08; optimized for cloning methylated or large DNA fragments.

Transformation Efficiency

Strain Efficiency
HB101 >1 × 10⁸ cfu/µg pBR322
JM109 >1 × 10⁸ cfu/µg pBR322
CJ236 >1 × 10⁷ cfu/µg pUC119
BMH71-18 mutS >1 × 10⁷ cfu/µg pUC119
MV1184 >1 × 10⁷ cfu/µg pUC119
HST02 >1 × 10⁸ cfu/µg pUC19
DH5α >1 × 10⁸ cfu/µg pUC19
HST08 >1 × 10⁸ cfu/µg pUC19
HST16CR >1 × 10⁸ cfu/µg pUC19

Stability of F` Plasmid and α-Complementation

  • JM109, BMH71-18 mutS, MV1184, HST02: Less than 1% white colonies when transformed with pUC19 or pUC119 and plated on L-agar with ampicillin, IPTG, and X-Gal.
  • CJ236: Strains lacking F` plasmid (pCJ105) do not grow on chloramphenicol plates.
  • DH5α: Blue colonies appear when transformed with pUC19 and plated on L-agar with ampicillin and X-Gal.

Additional Product Information

Refer to the product’s Certificate of Analysis for storage conditions, components, and technical specifications. Kit Components List and Certificates of Analysis are available under the Documents tab.

Takara Bio USA, Inc.
United States/Canada: +1.800.662.2566
Asia Pacific: +1.650.919.7300
Europe: +33.(0)1.3904.6880
Japan: +81.(0)77.565.6999

FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
© 2023 Takara Bio Inc. All Rights Reserved. All trademarks are the property of Takara Bio Inc. or its affiliates.

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