Takara Mighty TA-Cloning Kit

The Mighty TA-Cloning Kit is a fast, easy system for cloning PCR-amplified products obtained using a _Taq_-based DNA polymerase. Most DNA fragments amplified this way have a single base—deoxyriboadenosine (dA)—added to the 3’ end. The TA cloning method takes advantage of complementarity between this A-overhang on the insert and a single deoxyribothymidine (dT) overhang present on the 3’ end of a T-vector.

This kit includes a pUC-based T-vector, plus the same ligation reagents found in the DNA Ligation Kit, Mighty Mix, enabling highly efficient ligations to be performed simply and quickly.

More Information

Applications

• Fast, easy TA cloning of PCR products with 3’ A-overhangs, as generated with _Taq_-based DNA polymerases

Storage

–20°C

Additional product information

Please see the product`s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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