Thermo Fisher Scientific BMP-7 Polyclonal Antibody, Biotin, PeproTech

Applications

Western Blot (WB)

• Tested Dilution: 0.1–0.2 µg/mL

ELISA

• Tested Dilution: 0.25–1.0 µg/mL

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
MANVAENSSS DQRQACKKHE LYVSFRDLGW QDWIIAPEGY AAYYCEGECA FPLNSYMNAT NHAIVQTLVH FINPETVPKP CCAPTQLNAI SVLYFDDSSN VILKKYRNMV VRACGCH.

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human BMP-7. Anti-Human BMP-7-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect hBMP-7 by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human BMP-7 (500-P198) as a capture antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant hBMP-7.

Western Blot:
To detect Human BMP-7 by Western Blot analysis, use at 0.1–0.2 µg/mL. In conjunction with compatible secondary reagents, the detection limit for Recombinant Human BMP-7 is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.

Package Information:
500-P198BT-1MG will be provided as 2 × 500 µg.

Target Information

The bone morphogenetic proteins (BMPs) are a family of secreted signaling molecules that can induce ectopic bone growth. Many BMPs are part of the transforming growth factor-beta (TGFB) superfamily. BMPs were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extra-skeletal site. Based on its expression early in embryogenesis, BMP7 has a proposed role in early development. BMP7 is closely related to BMP5 and BMP6, suggesting possible bone inductive activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.