Thermo Fisher Scientific KGF Polyclonal Antibody, Biotin, PeproTech

Applications

Western Blot (WB)

• Tested Dilution: 0.1–0.2 µg/mL

ELISA

• Tested Dilution: 0.25–1.0 µg/mL
• Used in sandwich ELISA for detection of hKGF (FGF-7)

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
CNDMTPEQMA TNVNCSSPER HTRSYDYMEG GDIRVRRLFC RTQWYLRIDK RGKVKGTQEM KNNYNIMEIR TVAVGIVAIK GVESEFYLAM NKEGKLYAKK ECNEDCNFKE LILENHYNTY ASAKWTHNGG EMFVALNQKG IPVRGKKTKK EQKTAHFLPM AIT.

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human KGF (FGF-7). Anti-Human KGF (FGF-7)-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect hKGF by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human KGF (500-P19) as a capture antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant hKGF.

Western Blot:
To detect Human KGF by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used with compatible secondary reagents, the detection limit for Recombinant Human KGF is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.

Package:
500-P19BT-1MG will be provided as 2 × 500 µg.

Target Information

Plays an important role in the regulation of embryonic development, cell proliferation, and cell differentiation. Required for normal branching morphogenesis. Growth factor active on keratinocytes. Possible major paracrine effector of normal epithelial cell proliferation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.