Thermo Fisher Scientific HSP90AB1 (Heat Shock Protein 90) Monoclonal Antibody (HSP90AB1, 3953)
HSP90AB1 단백질을 인식하는 Thermo Fisher Scientific의 마우스 단일클론 항체로, Western Blot, IHC, Peptide Array 등에 사용 가능. 고순도 Protein A/G 정제, PBS 완충액 내 0.05% BSA 및 sodium azide 포함. 연구용으로만 사용.
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Thermo Fisher Scientific HSP90AB1 (Heat Shock Protein 90) Monoclonal Antibody (HSP90AB1, 3953)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | HSP90AB1, 3953 |
| Immunogen | Recombinant human HSP90AB1 protein fragment (around aa581–704) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunohistochemistry (PFA fixed): Incubate antibody for 30 minutes at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
Heat shock proteins (HSPs) are expressed in response to various biological stresses, including heat.
HSP90 is a 90 kDa protein that is induced under stress conditions but also one of the most abundant cellular proteins under non-stress conditions.
HSP90 proteins are highly conserved molecular chaperones involved in signal transduction, protein folding, degradation, and morphologic evolution.
They associate with cochaperones and help in folding newly synthesized proteins or stabilizing/refolding denatured proteins after stress.
HSP90 interacts with several intracellular proteins, including steroid receptors, actin, tubulin, aryl hydrocarbon (Ah) receptor, and kinases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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