Thermo Fisher Scientific Acidic Cytokeratin Monoclonal Antibody (OTI3E10), TrueMAB
Human reactive monoclonal antibody for acidic cytokeratin detection. Suitable for IHC (Paraffin) at 1:150 dilution. Lyophilized, unconjugated form with high purity via affinity chromatography. Ideal for epithelial marker studies and cancer research.
- 카탈로그번호
- CF190229
- 판매단위
- pk
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Applications
- Immunohistochemistry (Paraffin) (IHC (P)): Tested dilution 1:150
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rat / IgG |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI3E10 |
| Immunogen | Synthetic peptide corresponding to the conserved region of human type I (Acidic) keratins (including KRT9–KRT20) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Synthetic peptide sequence: EKVTMQNLND RLASYLDKVR ALEEAN
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
Cytokeratin pan belongs to the intermediate filament protein family, exhibiting significant biochemical diversity.
In human epithelial tissues, at least 20 distinct cytokeratin polypeptides (numbered 1–20) are expressed, with molecular weights ranging from 40–68 kDa and isoelectric points between 4.9–7.8.
Cytokeratin expression patterns vary by epithelial type, differentiation stage, and disease state, making them valuable markers in cancer research and tumor diagnosis.
These antibodies assist in differential tumor diagnosis via immunohistochemistry, cytopathology, and flow cytometry.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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