Thermo Fisher Scientific BrdU Monoclonal Antibody (OTI2B5), TrueMAB
BrdU에 특이적인 단클론 항체로 세포 증식 연구에 활용 가능. IHC, ICC/IF, Flow Cytometry에 적합. Mouse IgG1 형으로 고순도 정제. Lyophilized 형태로 안정적 보관 가능. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:150 |
| Flow Cytometry (Flow) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Chemical |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2B5 |
| Immunogen | Bromodeoxyuridine coupled to keyhole limpet hemocyanin (KLH) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
Bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU) is a synthetic nucleoside and thymidine analogue. It is widely used for detecting proliferating cells in living tissues by incorporation into newly synthesized DNA during the S phase of the cell cycle.
Antibodies specific for BrdU enable detection of incorporated BrdU, indicating cells actively replicating DNA. Binding requires DNA denaturation, typically through acid or heat exposure.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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