Thermo Fisher Scientific GL7 Monoclonal Antibody (GL-7 (GL7)), eFluor 450, eBioscience
GL7 단클론 항체(eFluor 450)는 활성화된 T/B 림프구 및 생쥐 생식중심 B세포 표지에 사용됩니다. 405/445 nm 형광 특성을 가지며 Flow Cytometry에 최적화되어 있습니다. 0.2 mg/mL 농도의 액상형으로 4°C 암저장 조건을 권장합니다.
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Thermo Fisher Scientific GL7 Monoclonal Antibody (GL-7 (GL7)), eFluor 450, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 3 publications |
| Immunocytochemistry (ICC/IF) | - | View 2 publications |
| Flow Cytometry (Flow) | 0.5 µg/test | View 31 publications |
| Functional Assay | - | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Rat / IgM |
| Recommended Isotype Control | Rat IgM Isotype Control (eBRM), eFluor™ 450, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | GL-7 (GL7) |
| Conjugate | eFluor™ 450 |
| Excitation/Emission Max | 405/445 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10854881 |
Available Formats
Product Specific Information
This GL7 monoclonal antibody reacts with a cell-surface protein found on T and B lymphocytes activated in vitro, on bone marrow pre-B-II cells, germinal center B cells, and also human B cell lines Ramos and Daudi. There is strain variability with respect to antigen distribution on thymocytes and Con A-activated spleen cells, with expression in BALB/c greater than that in C57BL/6. GL7 is commonly used as a marker for mouse germinal center B cells. The epitope of GL7 has been identified as a sialic acid glycan moiety called Neu5Ac, recognized by CD22.
- Applications Reported: Flow cytometric analysis
- Applications Tested: Flow cytometric analysis of stimulated mouse splenocytes (≤0.5 µg/test)
- Test definition: antibody amount to stain 100 µL cell sample (10⁵–10⁸ cells/test)
- Antibody titration recommended for optimal performance
eFluor® 450 Characteristics:
- Alternative to Pacific Blue®
- Emission: 445 nm
- Excitation: 405 nm (Violet Laser)
- Ensure instrument compatibility
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
GL7 is a cell-surface protein found on activated T and B lymphocytes, bone marrow pre-B-II cells, germinal center B cells, and human B cell lines (Ramos, Daudi). Expression varies by mouse strain (BALB/c > C57BL/6). GL7 epitope is Neu5Ac, a sialic acid glycan moiety recognized by CD22.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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