Thermo Fisher Scientific GL7 Monoclonal Antibody (GL-7 (GL7)), eFluor 450, eBioscience

Thermo Fisher Scientific GL7 Monoclonal Antibody (GL-7 (GL7)), eFluor 450, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488
• Biotin
• eFluor 660
• PE
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

This GL7 monoclonal antibody reacts with a cell-surface protein found on T and B lymphocytes activated in vitro, on bone marrow pre-B-II cells, germinal center B cells, and also human B cell lines Ramos and Daudi. There is strain variability with respect to antigen distribution on thymocytes and Con A-activated spleen cells, with expression in BALB/c greater than that in C57BL/6. GL7 is commonly used as a marker for mouse germinal center B cells. The epitope of GL7 has been identified as a sialic acid glycan moiety called Neu5Ac, recognized by CD22.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Flow cytometric analysis of stimulated mouse splenocytes (≤0.5 µg/test)
  • Test definition: antibody amount to stain 100 µL cell sample (10⁵–10⁸ cells/test)
  • Antibody titration recommended for optimal performance

eFluor® 450 Characteristics:

• Alternative to Pacific Blue®
• Emission: 445 nm
• Excitation: 405 nm (Violet Laser)
• Ensure instrument compatibility
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

GL7 is a cell-surface protein found on activated T and B lymphocytes, bone marrow pre-B-II cells, germinal center B cells, and human B cell lines (Ramos, Daudi). Expression varies by mouse strain (BALB/c > C57BL/6). GL7 epitope is Neu5Ac, a sialic acid glycan moiety recognized by CD22.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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