Thermo Fisher Scientific TER-119 Monoclonal Antibody (TER-119), eFluor 450, eBioscience
쥐 적혈구 계열 세포를 인식하는 TER-119 단클론 항체로, eFluor 450 형광 표지되어 흐름세포분석에 적합합니다. 405 nm 레이저로 여기되어 445 nm에서 방출하며, 0.5 µg/test 농도로 사용 권장됩니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 6 publications |
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| Flow Cytometry (Flow) | 0.5 µg/test | View 88 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Fish, Human, Mouse |
| Host / Isotype | Rat / IgG2b, kappa |
| Recommended Isotype Control | Rat IgG2b kappa Isotype Control (eB149/10H5), eFluor™ 450, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | TER-119 |
| Conjugate | eFluor™ 450 |
| Excitation/Emission Max | 405/445 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1518809 |
Available Formats
- Unconjugated
- Alexa Fluor 660
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 395 / 615 / 661 / 737 / 805
- Brilliant Violet 421 / 480
- eFluor 506
- FITC
- Functional Grade
- PE / PE-Cyanine5 / PE-Cyanine5.5 / PE-Cyanine7 / PE-eFluor 610
- PerCP-Cyanine5.5 / PerCP-eFluor 710
- Super Bright 436 / 600 / 645 / 702 / 780
- Request custom conjugation
Product Specific Information
The TER-119 monoclonal antibody reacts with mouse erythroid cells from early proerythroblast to mature erythrocyte stages.
The TER-119 antigen is present in yolk sac, fetal and newborn liver, but is not expressed by cells carrying BFU-E and CFU-E activities.
Several erythroleukemia cell lines tested so far are negative for TER-119 expression even after dimethylsulfoxide stimulation.
Biochemical and molecular analyses indicate that the TER-119 antigen is associated with surface glycophorin A but is not a typical glycophorin.
- Applications Reported: Flow cytometric analysis
- Applications Tested: Flow cytometry of mouse bone marrow cells
- Recommended ≤ 0.5 µg/test
- A test is defined as the amount of antibody used in 100 µL final volume
- Cell number: 10⁵–10⁸ cells/test (empirically determined)
- Titrate for optimal performance
Fluorophore Information:
eFluor® 450 is an alternative to Pacific Blue®.
- Excitation: 405 nm
- Emission: 445 nm
- Laser: Violet
- Ensure instrument compatibility.
- Filtration: 0.2 µm post-manufacturing filtered.
Target Information
TER-119 is a lineage marker for erythroid cells from early proerythroblast to mature erythrocyte stages in adult blood, spleen, and bone marrow.
It is also present in yolk sac, and fetal and newborn liver.
The TER-119 antigen is not expressed by cells at earlier erythroid development stages (BFU-E, CFU-E).
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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