Thermo Fisher Scientific CD93 (AA4.1) Monoclonal Antibody (AA4.1), eFluor 450, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 0.5 µg/test
• Publications: View 6 publications

Product Specifications

Available Formats

• APC
• Biotin
• FITC
• PE
• PE-Cyanine7
• PerCP-Cyanine5.5
• Super Bright 436
• Super Bright 600
• Super Bright 702
• Request custom conjugation

Product Specific Information

The AA4.1 monoclonal antibody reacts with mouse C1qRp, an approximately 130–140 kDa C-type lectin-like type I transmembrane protein. This antigen is expressed on early stages of B cell development in bone marrow, and also detectable in adult spleen and fetal liver.
In combination with markers such as Thy-1, Sca-1, c-Kit, CD43, and CD24, it helps distinguish primitive and differentiated subsets of Lymphoid-Committed Progenitors (CLP).

Surface expression of IgM, CD23, and AA4.1 defines three transitional B cell subsets:

• T1: AA4.1⁺ / CD23⁻ / sIgM^hi
• T2: AA4.1⁺ / CD23⁺ / sIgM^hi
• T3: AA4.1⁺ / CD23⁺ / sIgM^lo

AA4.1 is also found in endothelial cells, megakaryoblasts, and platelets. Antibodies AA4.1 and 493 recognize different epitopes of the same molecule.

Applications Reported: Flow cytometric analysis
Applications Tested: Flow cytometric analysis of mouse bone marrow cells (≤0.5 µg/test).
Recommended to titrate antibody for optimal performance.

Fluorophore Information:
eFluor® 450 is an alternative to Pacific Blue®, excited at 405 nm and emitting at 445 nm. Ensure instrument compatibility.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The encoded protein is a cell-surface glycoprotein and type I membrane protein originally identified as a myeloid cell-specific marker. It is believed to be involved in intercellular adhesion and apoptotic cell clearance. The cytoplasmic tail interacts with moesin, linking to the cytoskeleton and participating in cytoskeletal remodeling.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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