Thermo Fisher Scientific KLRG1 Monoclonal Antibody (2F1), eFluor 450, eBioscience
마우스 KLRG1 단백질을 인식하는 단클론 항체로, Flow Cytometry에 최적화되어 있습니다. eFluor 450 형광체로 결합되어 405/445 nm 파장에서 검출됩니다. 4°C 암소 보관하며, 0.2 µm 필터로 여과된 액상 제품입니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Flow Cytometry (Flow) | 0.25 µg/test | View 23 publications |
| Functional Assay (Functional) | - | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Syrian hamster / IgG |
| Recommended Isotype Control | Syrian Hamster IgG Isotype Control, eFluor™ 450, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2F1 |
| Conjugate | eFluor™ 450 |
| Excitation/Emission Max | 405 / 445 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10852843 |
Additional Available Formats
- Alexa Fluor 488
- Alexa Fluor 532
- Alexa Fluor 561
- Alexa Fluor 647
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 395
- Brilliant UV 496
- Brilliant UV 563
- Brilliant UV 615
- Brilliant Violet 421
- Brilliant Violet 480
- Brilliant Violet 605
- Brilliant Violet 711
- eFluor 506
- FITC
- Functional Grade
- Pacific Orange
- PE
- PE-Cyanine5
- PE-Cyanine5.5
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Super Bright 645
- Super Bright 702
- Super Bright 780
- Request custom conjugation
Product Specific Information
The 2F1 monoclonal antibody reacts with mouse Killer cell Lectin-like Receptor G1 (KLRG1), also known as Mast cell Function-associated Antigen (MAFA). KLRG1 is a glycosylated homodimer (30–38 kDa) containing an ITIM-like cytoplasmic motif. It is expressed on approximately one-third of mouse NK cells and a subset of T cells. MHC class I molecules regulate KLRG1 expression through interactions with Ly49 molecules and SHP-1 signaling.
Applications Reported: Flow cytometric analysis.
Applications Tested: Tested using mouse splenocytes at ≤0.25 µg/test (100 µL final volume). Optimal titration recommended.
Fluorophore Information:
eFluor® 450 is an alternative to Pacific Blue®, excited at 405 nm and emitting at 445 nm. Ensure instrument compatibility.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
KLRG1 (Killer cell lectin-like receptor G1, MAFA, 2F1-Ag) is a homodimeric lectin-like type 2 transmembrane receptor containing ITIM motifs that interact with SH2 domains of phosphatases such as SHP-1. It functions as an inhibitory receptor expressed on NK cells and certain T cells, contributing to immune regulation, tumor cell lysis, and antiviral responses.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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