Thermo Fisher Scientific IFN gamma Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-0.2 µg/mL

View 2 publications 2 publications

Immunohistochemistry (IHC)

5-15 µg/mL

View 1 publication 1 publication

Neutralization (Neu)

0.08-0.20 µg/mL

-

Inhibition Assays (IA)

0.08-0.20 µg/mL

-

Product Specifications

Species Reactivity

Mouse, Rat

Published species

Mouse

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Purified, recombinant rat IFNG, expressed in E. coli.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 5% trehalose

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_794536

Product Specific Information

PA1-24782 will not react with human.

The Neutralization Dose 50 (ND50) for anti-rat IFNG is approximately 0.08 to 0.2 µg/mL in the presence of 2.5 ng/mL of recombinant rat IFNG, measuring the protection of mouse L-929 cells from the lytic effect of the virus.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor, and immune interferon that is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a dimeric protein consisting of two 146 amino acid subunits. IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS-PAGE, IFN gamma appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma homodimer is highly species specific. Human IFN gamma does not show cross-reactivity with mouse. IFN gamma function includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes. IFN gamma is involved in cytokine regulation and also acts synergistically with other cytokines. Activation of IFN gamma takes place through binding of IFN gamma receptor I and II, and activating the JAK-STAT pathway. IFN gamma does not show any homology with IFN alpha or IFN beta but human IFN gamma shows about 40% sequence homology with mouse IFN gamma. IFN gamma is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. IFN gamma gene mutations are associated with aplastic anemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.