Thermo Fisher Scientific IFN gamma Polyclonal Antibody
Thermo Fisher Scientific의 IFN gamma Polyclonal Antibody는 Mouse 및 Rat 시료에 반응하며, Western blot, IHC, Neutralization, Inhibition Assay 등에 사용됩니다. Goat IgG 기반의 비결합형 항체로, 항원 친화 크로마토그래피로 정제되었습니다. 장기 보관 시 -20°C에서 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL | View 2 publications |
| Immunohistochemistry (IHC) | 5–15 µg/mL | View 1 publication |
| Neutralization (Neu) | 0.08–0.20 µg/mL | - |
| Inhibition Assays (IA) | 0.08–0.20 µg/mL | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Mouse, Rat |
| Published Species | Mouse |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Purified, recombinant rat IFNG, expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 5% trehalose |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_794536 |
Product Specific Information
- PA1-24782 will not react with human.
- The Neutralization Dose 50 (ND50) for anti-rat IFNG is approximately 0.08–0.2 µg/mL in the presence of 2.5 ng/mL recombinant rat IFNG, measuring protection of mouse L-929 cells from viral lysis.
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor and immune interferon produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, and other cytokines.
It is a dimeric glycoprotein composed of two 146 amino acid subunits (~45 kDa functional homodimer).
On SDS-PAGE, IFN gamma appears as 25, 20, and minor 15.5 kDa bands due to differential glycosylation.
Functional roles include:
- Antiviral and tumor antiproliferative activity
- Induction of class I and II MHC
- Macrophage activation
- Enhanced immunoglobulin secretion by B lymphocytes
- Cytokine regulation and synergistic action with other cytokines
Activation occurs through binding of IFN gamma receptor I and II, activating the JAK-STAT pathway.
Human IFN gamma shows ~40% sequence homology with mouse IFN gamma and does not cross-react.
Gene mutations are associated with aplastic anemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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