Thermo Fisher Scientific IL5RA (soluble) Polyclonal Antibody
Human IL5RA(IL-5 receptor alpha) 인식하는 Goat Polyclonal Antibody로, WB, Flow Cytometry, Neutralization, Inhibition Assay에 사용 가능. 항원 친화 크로마토그래피로 정제되었으며, 무보존액 PBS buffer에 보관. 연구용으로만 사용.
- 판매단위
- pk
카탈로그
1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific IL5RA (soluble) Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL |
| Flow Cytometry (Flow) | 2.5 µg / 10⁶ cells |
| Neutralization (Neu) | 1–3 µg/mL |
| Inhibition Assays (IA) | 1–3 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Purified, recombinant human interleukin 5 soluble receptor alpha (IL5RA) expressed in insect Sf21 cells |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 5% trehalose |
| Contains | No preservative |
| Storage Conditions | Store at 4°C for short term; for long term storage, store at -20°C avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_779598 |
Product Specific Information
- Recommended positive controls: human blood-derived granulocytes
- Neutralization Dose₅₀ (ND₅₀) for anti-human IL5RA is approximately 1–3 µg/mL in the presence of 0.5 ng/mL recombinant human IL-5 using the human TF-1 cell line.
- ND₅₀ indicates the antibody concentration required to achieve half-maximal inhibition of IL5RA-mediated IL5 response on responsive cells.
- The exact neutralization concentration depends on IL5 concentration and IL5RA expression level on the cell surface.
Target Information
Interleukin 5 (IL-5) is a soluble T cell-derived factor, also known as T cell replacing factor (TRF), that induces B cell and eosinophil growth and differentiation.
IL-5 acts through the IL-5 receptor (IL-5R), composed of two chains:
- Alpha chain (IL5RA): binds IL-5 with low affinity
- Beta chain: forms a high-affinity receptor complex with the alpha chain
The cytoplasmic domains of both subunits are essential for signal transduction. The membrane-proximal proline-rich sequence of IL5RA is critical for IL-5–induced proliferation, proto-oncogene expression, and tyrosine phosphorylation of proteins such as JAK1 and JAK2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.