Thermo Fisher Scientific Cdc27 Monoclonal Antibody (AF3.1)
Cdc27 단백질을 인식하는 AF3.1 클론의 Mouse 모노클로날 항체로, WB, IHC, ICC, ELISA, IP 등 다양한 응용에 사용 가능. HeLa 핵 추출물 양성 대조로 권장. Protein A 정제, PBS 버퍼에 보존, 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1–2 µg/mL | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent | - |
| Immunocytochemistry (ICC/IF) | Assay-dependent | - |
| ELISA | Assay-dependent | - |
| Immunoprecipitation (IP) | Assay-dependent | View 1 publication |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Bovine, Dog, Human, Mouse, Rat, Xenopus |
| Published Species | Mouse |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | AF3.1 |
| Immunogen | Synthetic peptide conjugated to KLH, corresponding to residues 814–823 of human Cdc27 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 2.3 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 15 mM sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2075283 |
Product Specific Information
- Recommended positive control: HeLa nuclear extract
- Store product as a concentrated solution.
- Centrifuge briefly prior to opening the vial.
Target Information
The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae protein Cdc27 and the gene product of Schizosaccharomyces pombe nuc2.
This protein is a component of the anaphase-promoting complex (APC), composed of eight subunits conserved in eukaryotic cells.
APC catalyzes the formation of cyclin B-ubiquitin conjugates responsible for ubiquitin-mediated proteolysis of B-type cyclins.
Cdc27 and other APC complex members contain the TPR (tetratricopeptide repeat) domain, crucial for protein-protein interactions.
It interacts with mitotic checkpoint proteins including Mad2, p55CDC, and BUBR1, contributing to mitotic timing control.
Alternatively spliced transcript variants encoding different isoforms have been identified for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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