Thermo Fisher Scientific MDM2 Monoclonal Antibody (HDM2-323)

Applications and Tested Dilution

Product Specifications

Product Specific Information

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

MDM2 is a ubiquitin ligase for p53 and plays a central role in the regulation of p53 stability. It is located on chromosome 12q. Akt-mediated phosphorylation of MDM2 at Ser166 and Ser186 increases its interaction with p300, allowing MDM2-mediated ubiquitination and degradation of p53. Phosphorylation also blocks its binding to p19ARF, further increasing p53 degradation.

MDM2 negatively regulates p53 by inhibiting its transactivation function and promoting its degradation via E3 ubiquitin ligase activity. Overexpression of MDM2 can lead to inactivation of p53 and reduced tumor suppressor function. MDM2 is involved in apoptosis and cell cycle regulation and is overexpressed in various human malignancies including soft tissue carcinomas and breast cancer. It also interacts with proteins such as retinoblastoma 1 and ribosomal protein L5, contributing to cell cycle, apoptosis, and tumorigenesis. More than 40 alternatively spliced variants of MDM2 have been identified in both tumor and normal tissues.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.