Thermo Fisher Scientific CD39 Monoclonal Antibody (eBioA1 (A1)), eBioscience
CD39 단백질을 인식하는 eBioA1 (A1) 단클론 항체로, 인간 및 생쥐 시료에 반응합니다. Flow cytometry, IHC, WB 등 다양한 응용에 적합하며, 0.25 µg/test로 사용 가능합니다. PBS 완충액(0.09% sodium azide) 내 액상 형태로 제공되며, 4°C에서 보관합니다.
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- 14-0399-xx (3개 옵션)
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카탈로그
3개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD39 Monoclonal Antibody (eBioA1 (A1)), eBioscience
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1 µg/mL | - |
| Immunohistochemistry (IHC) | - | 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1 µg/mL | - |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-Dependent | 1 publication |
| Immunocytochemistry (ICC/IF) | - | 1 publication |
| Flow Cytometry (Flow) | 0.25 µg/test | 15 publications |
| ELISA | - | 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBioA1 (A1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2864874 |
Available Conjugated Formats
- APC
- APC-eFluor 780
- Brilliant UV 805
- FITC
- NovaFluor Blue 610-70S
- NovaFluor Blue 660-120S
- NovaFluor Blue 690
- NovaFluor Red 700
- NovaFluor Red 725
- NovaFluor Red 755
- NovaFluor Yellow 660
- NovaFluor Yellow 755
- PE
- PE-Cyanine7
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Super Bright 702
- Request custom conjugation available
Product Specific Information
Description:
The eBioA1 monoclonal antibody reacts with human CD39 (ectonucleoside triphosphate diphosphohydrolase 1, ENTPD1, or NTPDase). CD39 is an integral membrane protein with two transmembrane domains and exists as a homotetramer. It acts as the main ectoenzyme of the immune system, converting extracellular ATP to ADP or AMP, often functioning with CD73 to hydrolyze ATP. It is expressed on activated lymphocytes, subsets of T cells, B cells, dendritic cells, and weakly on monocytes and granulocytes.
CD39 and CD73 are also expressed on regulatory T cells (Treg), facilitating their migration into inflamed tissues with high extracellular ATP. Expression on Foxp3+CD4+ T cells ranges from 25–45%.
Applications Reported:
- Flow cytometric analysis
- Immunohistochemical staining
Applications Tested:
Tested by flow cytometric analysis of normal human peripheral blood cells. Recommended ≤0.25 µg/test (100 µL final volume). Cell number: 10⁵–10⁸ cells/test. Optimize titration for best results.
Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD39 is a 70–100 kDa cell surface antigen expressed on peripheral blood B cells, monocytes, T cell clones, and weakly on granulocytes. It has intrinsic ecto-ATPase activity and is induced on T cells and increased on B cells as a late activation antigen.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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