Thermo Fisher Scientific CD22 Monoclonal Antibody (eBio4KB128 (4KB128)), NovaFluor Blue 510, eBioscience

Thermo Fisher Scientific CD22 Monoclonal Antibody (eBio4KB128 (4KB128)), NovaFluor Blue 510

Applications

• Reported Application: Flow Cytometry
• Tested Dilution: 5 µL (0.2 µg)/test
• Publications: Not specified

Product Specifications

Available Formats:

• Unconjugated
• APC
• FITC
• NovaFluor Blue 690
• NovaFluor Red 755
• NovaFluor Yellow 755
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Request custom conjugation

Product Specific Information

Description:
The eBio4KB128 monoclonal antibody recognizes human CD22 (Siglec-2), a member of the siglec subgroup of the Ig superfamily. CD22 is a type I transmembrane glycoprotein composed of two polypeptide chains (CD22α and CD22β, 130–140 kDa) produced by alternative splicing. It is highly expressed on mature B cells and B cell lymphomas.

The extracellular portion contains seven Ig-like domains capable of binding sialic acid moieties on various cell types. The intracellular portion includes six tyrosine residues within ITIM and activation-like motifs phosphorylated upon B-cell receptor engagement, enabling regulation of B-cell receptor signaling.

Each product contains:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-diluted and tested on normal human peripheral blood cells. Recommended use: 5 µL (0.02 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test).

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Recommended viability dyes: Invitrogen LIVE/DEAD Fixable Dead Cell stains.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) is required for optimal staining.
  • Add 5 µL per sample before combining with cells.
  • For single-color controls: 5 µL per 100 µL cell sample (10³–10⁸ cells).

Technology:
NovaFluor conjugates utilize Phiton™ technology with engineered nucleic acid dye structures for optimized fluorescent signatures minimizing spillover and spread.

• Excitation: 496 nm
• Emission: 511 nm
• Laser: 488 nm (Blue)

Target Information

CD22 (BL-CAM) is a type I integral membrane glycoprotein (130–140 kDa) expressed in the cytoplasm and membrane of B-lymphocytes. It appears early in B-cell differentiation, similar to CD19. CD22 expression is limited to mature B cells and plasma cells, aiding in leukemia phenotyping.

Expression Profile:

• Strongly expressed in hairy cell leukemia
• Positive in diffuse large B-cell lymphoma and nodular lymphocyte predominance Hodgkin’s lymphoma
• Negative in classical Hodgkin’s lymphoma

CD22 preferentially binds α2,6-linked sialic acid. Ligand-induced tyrosine phosphorylation regulates B-cell antigen receptor signaling, acting in both positive and inhibitory pathways via Src family kinases and phosphatases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

제품 이미지