Thermo Fisher Scientific CD22 Monoclonal Antibody (eBio4KB128 (4KB128)), NovaFluor Blue 660-120S, eBioscience

Applications

Tested Application

• Flow Cytometry (Flow)

Tested Dilution

• 5 µL (0.2 µg)/test

Product Specifications

Additional Formats

• Unconjugated
• APC
• FITC
• NovaFluor Blue 690
• NovaFluor Red 755
• NovaFluor Yellow 755
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Request custom conjugation

Product Specific Information

Description:
The eBio4KB128 monoclonal antibody recognizes human CD22 (Siglec-2), a member of the siglec subgroup of the Ig superfamily. CD22 is a type I transmembrane glycoprotein composed of two polypeptide chains (CD22α, CD22β; 130 and 140 kDa) produced by alternative splicing. It is highly expressed on mature B cells and B cell lymphomas. The extracellular region contains seven Ig-like domains capable of binding sialic acid ligands, while the intracellular region contains ITIM and activation-like motifs that regulate B-cell receptor signaling.

Each product contains 1 vial of NovaFluor conjugate and 1 vial of CellBlox Plus Blocking Buffer.

Applications Reported:

• Reported for use in flow cytometric analysis.

Applications Tested:

• Pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells.
• Recommended use: 5 µL (0.2 µg) per test in a final volume of 100 µL.
• Cell number range: 10⁵–10⁸ cells/test.

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes such as propidium iodide, 7-AAD, and DAPI.
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for NovaFluor dyes.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) is required for optimal staining.
  • Add 5 µL per sample to antibody cocktails before combining with cells.
  • For single-color controls: 5 µL per 100 µL cell sample (10³–10⁸ cells).

NovaFluor Conjugate Information:
NovaFluor conjugates are based on Phiton™ technology utilizing novel nucleic acid dye structures engineered for optimal spectral properties and minimal spillover.

• Excitation: 509 nm
• Emission: 665 nm
• Compatible Laser: 488 nm (Blue)

Target Information

CD22 (BL-CAM) is a type I integral membrane glycoprotein (130–140 kDa) expressed in the cytoplasm and membrane of B-lymphocytes. It appears early in B-cell differentiation, similar to CD19, and is expressed in mature B cells but absent in plasma cells. CD22 is useful for phenotyping mature leukemias and is strongly expressed in hairy cell leukemia, diffuse large B-cell lymphoma, and nodular lymphocyte predominance Hodgkin’s lymphoma (negative in classical Hodgkin’s lymphoma).
It binds preferentially to α2,6-linked sialic acid and participates in B-cell receptor signaling through phosphorylation of tyrosine residues, acting in both activation and inhibition pathways.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.

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