Thermo Fisher Scientific CD22 Monoclonal Antibody (eBio4KB128 (4KB128)), NovaFluor Red 755, eBioscience

Thermo Fisher Scientific CD22 Monoclonal Antibody (eBio4KB128 (4KB128)), NovaFluor Red 755

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.2 µg)/test

Product Specifications

Additional Formats

• Unconjugated
• APC
• FITC
• NovaFluor Blue 690
• NovaFluor Yellow 755
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Request custom conjugation

Product Specific Information

The eBio4KB128 monoclonal antibody recognizes human CD22 (Siglec-2), a member of the siglec subgroup of the Ig superfamily. CD22 is a type I transmembrane glycoprotein composed of two polypeptide chains (CD22α and CD22β, 130 and 140 kDa) produced by alternative splicing. It is highly expressed on mature B cells and B cell lymphomas.

The extracellular portion contains seven Ig-like domains capable of binding sialic acid-containing ligands, while the intracellular region includes tyrosine residues within ITIM motifs that regulate B-cell receptor signaling.

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and tested on normal human peripheral blood cells at 5 µL (0.2 µg)/test.
A test corresponds to staining a cell sample (10⁵–10⁸ cells) in 100 µL final volume.

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Important Notes:

• NovaFluor dyes are incompatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability assays.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) is required for optimal staining.
• Add 5 µL per sample to antibody cocktails or single-color controls (10³–10⁸ cells).

NovaFluor conjugates utilize Phiton™ technology, featuring engineered fluorescent signatures to minimize spillover and spread.

Excitation: 636 nm
Emission: 756 nm
Laser: 633–640 nm (Red)

Target Information

CD22 (BL-CAM) is a type I integral membrane glycoprotein (130–140 kDa) expressed in B-lymphocytes. It appears early in B-cell differentiation and is limited to mature B cells and plasma cells, aiding in leukemia phenotyping. CD22 binds α2,6-linked sialic acid and participates in B-cell receptor signaling regulation through phosphorylation and phosphatase recruitment. It is strongly expressed in hairy cell leukemia, diffuse large B-cell lymphoma, and nodular lymphocyte predominance Hodgkin’s lymphoma, but negative in classical Hodgkin’s lymphoma.

For Research Use Only. Not for use in diagnostic procedures.

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