Thermo Fisher Scientific STIM1 Polyclonal Antibody
STIM1 단백질을 인식하는 Sheep Polyclonal Antibody로, WB, IHC, ELISA, IP에 사용 가능. 인간 시료에 반응하며, Affinity chromatography로 정제됨. 액상 형태로 -20°C 보관. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 5 µg/mL |
| ELISA | 1:10,000 |
| Immunoprecipitation (IP) | 1 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Sheep / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Stim1 affinity purified antibody prepared from whole sheep serum produced by repeated immunizations with a synthetic peptide near the N-terminus of human Stim1. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.12 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 1 mg/mL BSA, 50% glycerol |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid repeated freeze-thaw cycles. Centrifuge product if not completely clear after standing at room temperature.
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
A BLAST analysis suggests cross-reactivity with rat, human, and mouse based on 100% sequence homology. Cross-reactivity with STIM1 from other sources has not been determined.
Target Information
STIM1 (stromal interacting molecule; also known as GOK) acts as a sensor of calcium depletion within the endoplasmic reticulum and transduces the signal to Orai1, the presumptive CRAC channel at the plasma membrane.
Following a decrease in luminal calcium concentration, STIM1 oligomerizes and induces Orai1 to enable entry of extracellular calcium into the cytoplasm. The precise mechanism of STIM1-Orai1 interaction has not yet been fully elucidated.
STIM1 associates with growing ends of microtubules and is involved in endoplasmic reticulum tubule extension.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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