Thermo Fisher Scientific Cyanine Polyclonal Antibody, Rhodamine

Applications

• Western Blot (WB): 1:1,000
• Immunocytochemistry (ICC/IF): 1:500–1:2,500

Product Specifications

Product Specific Information

Store vial at 4°C prior to restoration. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
Stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Sheep Serum and cyanine conjugated IgG. Reactivity was observed against Cy2, Cy3 and Cy5. Reactivity against other cyanine analogs is unknown.

Target Information

Cyanines are blue-green fluorescent dyes belonging to the polymethine group, used in biotechnology for labeling and analysis. The spectrum for Cyanine ranges from IR (489/506) to UV (743/767).
Cyanine dyes include Cy2, Cy3, Cy3B, Cy3.5, Cy5, Cy5.5, and Cy7. These dyes exhibit brighter and more stable fluorescence than traditional FITC or TRITC/Rhodamine dyes.
Cy3 and Cy5 are most commonly used for two-color detection in Flow Cytometry applications.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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