Thermo Fisher Scientific Cyanine Polyclonal Antibody, Rhodamine
Thermo Fisher Scientific의 Cyanine Polyclonal Antibody는 Rhodamine으로 결합된 sheep IgG 기반 항체로 Cy2, Cy3, Cy5에 반응합니다. Western blot과 Immunocytochemistry에 적합하며, 높은 형광 안정성과 밝기를 제공합니다. 연구용으로만 사용됩니다.
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Applications
- Western Blot (WB): 1:1,000
- Immunocytochemistry (ICC/IF): 1:500–1:2,500
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Chemical |
| Host/Isotype | Sheep / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A mixture of Cy3 and Cy5 conjugated KLH |
| Conjugate | Rhodamine |
| Excitation/Emission Max | 573/591 nm |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 10 mg/mL BSA |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C (avoid freeze/thaw cycles) |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at 4°C prior to restoration. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
Stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Sheep Serum and cyanine conjugated IgG. Reactivity was observed against Cy2, Cy3 and Cy5. Reactivity against other cyanine analogs is unknown.
Target Information
Cyanines are blue-green fluorescent dyes belonging to the polymethine group, used in biotechnology for labeling and analysis. The spectrum for Cyanine ranges from IR (489/506) to UV (743/767).
Cyanine dyes include Cy2, Cy3, Cy3B, Cy3.5, Cy5, Cy5.5, and Cy7. These dyes exhibit brighter and more stable fluorescence than traditional FITC or TRITC/Rhodamine dyes.
Cy3 and Cy5 are most commonly used for two-color detection in Flow Cytometry applications.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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