Thermo Fisher Scientific Cyanine Polyclonal Antibody, AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunohistochemistry (IHC)

1:200-1:1,000

-

ELISA (ELISA)

1:2,000-1:10,000

-

Immunoprecipitation (IP)

1:500-1:2,500

-

Dot blot (DB)

1:1,000

-

Product Specifications

Species Reactivity

Chemical

Host/Isotype

Sheep / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A mixture of Cy3 and Cy5 conjugated KLH

Conjugate

AP AP AP

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

0.05M tris HCl, pH 8, with 10mg/mL BSA, 0.15M NaCl, 0.0001M ZnCl2, 0.001M MgCl2, 50% glycerol

Contains

0.01% sodium azide

Storage conditions

4° C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Sheep Serum and cyanine conjugated IgG. Reactivity was observed against Cy2, Cy3 and Cy5. Reactivity against other cyanine analogs is unknown.

Target Information

Cyanines are blue-green fluorescent dyes belonging to polymethine group, used in biotechnology for labeling and analysis. The spectrum for Cyanine ranges from IR (489/506) to UV (743/767). Cyanine dyes include Cy2, Cy3, Cy3B, Cy3.5, Cy5, Cy5.5 and Cy7. These dyes exhibit brighter and more stable fluorescence than traditional FITC, TRITC/Rhodamine dyes. Cy3 and Cy5 are most commonly used for 2 color detection in Flow Cytometry applications.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.