Thermo Fisher Scientific Cyanine Polyclonal Antibody, FITC

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunocytochemistry (ICC/IF)

1:500-1:2,500

-

Product Specifications

Species Reactivity

Chemical

Host/Isotype

Sheep / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A mixture of Cy3 and Cy5 conjugated KLH

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Lyophilized

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

0.02M potassium phosphate, pH 7.2, with 10mg/mL BSA, 0.15M NaCl

Contains

0.01% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Store in the dark.

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-Sheep Serum and cyanine conjugated IgG. Reactivity was observed against Cy2, Cy3 and Cy5. Reactivity against other cyanine analog is unknown.

Target Information

Cyanines are blue-green fluorescent dyes belonging to polymethine group, used in biotechnology for labeling and analysis. The spectrum for Cyanine ranges from IR (489/506) to UV (743/767). Cyanine dyes include Cy2, Cy3, Cy3B, Cy3.5, Cy5, Cy5.5 and Cy7. These dyes exhibit brighter and more stable fluorescence than traditional FITC, TRITC/Rhodamine dyes. Cy3 and Cy5 are most commonly used for 2 color detection in Flow Cytometry applications.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.