Thermo Fisher Scientific BCL6 Monoclonal Antibody (BCL-DWN), PerCP-eFluor 710, eBioscience
마우스 Bcl-6 단백질을 인식하는 BCL-DWN 단클론 항체로, PerCP-eFluor 710 형광체가 결합된 제품입니다. Flow Cytometry용으로 최적화되어 있으며, 고감도 검출과 안정적인 형광 신호를 제공합니다. 4°C 암소 보관, 비동결 조건 유지.
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Thermo Fisher Scientific BCL6 Monoclonal Antibody (BCL-DWN), PerCP-eFluor 710, eBioscience
Applications
- Flow Cytometry (Flow)
Tested Dilution: 0.015 µg/test
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Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), PerCP-eFluor™ 710, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | BCL-DWN |
| Conjugate | PerCP-eFluor™ 710 |
| Excitation / Emission Max | 482 / 708 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2573767 |
Product Specific Information
This BCL-DWN monoclonal antibody reacts with mouse Bcl-6 (B-cell lymphoma 6), a transcriptional repressor abundantly expressed in germinal center B cells and activated B cells. Bcl-6 is essential for B cell proliferation and survival, and plays a role in pre-B cell survival during development. It is constitutively expressed in DLBCLs, Burkitt’s lymphoma, and non-Hodgkin’s lymphoma, as well as in CD4+ T follicular helper and memory T cells. Bcl-6 expression is antagonized by Blimp-1.
Applications Reported: Intracellular staining followed by flow cytometric analysis.
Applications Tested: Tested by flow cytometric analysis of splenocytes from SRBC-immunized mice. Recommended ≤0.015 µg/test (100 µL volume). Cell number range: 10⁵–10⁸ cells/test. Optimize by titration for best results.
Fluorophore Information:
PerCP-eFluor® 710 emits at 710 nm and is excited by a 488 nm blue laser. Recommended filter: 710/50 bandpass (695/40 acceptable). Ensure instrument compatibility.
Light Sensitivity: Protect vial and stained samples from light.
Fixation: Store samples up to 3 days in IC Fixation Buffer (Product #00-8222) or 1-step Fix/Lyse Solution (Product #00-5333) at 4°C in dark.
Excitation: 488 nm; Emission: 710 nm; Laser: Blue.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Bcl-6 proto-oncogene encodes a Kruppel-type zinc-finger protein mainly expressed in germinal center B cells and related lymphomas. It is involved in chromosome rearrangements at 3q27 in non-Hodgkin’s lymphomas, detected in 33–45% of diffuse large B cell lymphomas. Bcl-6 expression is observed in follicular, diffuse large B cell, and Burkitt’s lymphomas, as well as in nodular lymphocyte predominant Hodgkin’s disease.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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