Thermo Fisher Scientific ATF2 Recombinant Rabbit Monoclonal Antibody (SZ17-01)
Western blot, IHC, ICC/IF에 적합한 ATF2 단백질 검출용 재조합 토끼 단클론 항체. 높은 특이성과 민감도, 로트 간 일관성 제공. 인간 및 생쥐 시료에 반응하며, 장기 보관 시 -20°C에서 안정적 유지.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | SZ17-01 |
| Immunogen | Synthetic peptide within Human ATF2 (aa 456–505) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809316 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The specific antibody DNA sequences from immunoreactive rabbits are cloned and screened to select the best candidates for production.
Advantages include:
- Improved specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to the larger rabbit immune repertoire
Target Information
The transcription factor ATF-2 (also known as CRE-BP1) binds to both AP-1 and CRE DNA response elements and belongs to the ATF/CREB family of leucine zipper proteins.
ATF-2 interacts with viral oncoproteins and cellular tumor suppressors and is a target of the SAPK/JNK and p38 MAP kinase signaling pathways.
Cellular stress such as genotoxic agents, inflammatory cytokines, and UV irradiation stimulate ATF-2 transcriptional activity through phosphorylation at Thr69 and Thr71. Both SAPK and p38 MAPK phosphorylate ATF-2 at these sites in vitro and in transfected cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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