Thermo Fisher Scientific Caspase 8 Recombinant Rabbit Monoclonal Antibody (SZ01-08)
Caspase 8 표적의 재조합 토끼 단클론 항체로, 높은 특이성과 감도를 제공. Western blot, IHC, ICC/IF에 적합하며, 일관된 로트 간 품질 보장. 단백질 A 정제, 액상 형태로 제공되며, 장기 보관 시 -20°C 권장.
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Thermo Fisher Scientific Caspase 8 Recombinant Rabbit Monoclonal Antibody (SZ01-08)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:5,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | SZ01-08 |
| Immunogen | Synthetic peptide within Human Caspase-8 aa 207–256 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809317 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. These systems clone specific antibody DNA sequences from immunoreactive rabbits, followed by screening to select optimal production candidates.
Advantages include:
- Improved specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to the large rabbit immune repertoire
Target Information
Caspase 8 (CASP8) is a cysteine-aspartic acid protease involved in apoptosis. It exists as an inactive proenzyme and becomes active through proteolytic processing, forming a heterodimeric enzyme. Caspase 8 mediates Fas-induced cell death and interacts with FADD via death effector domains.
It has been detected in affected brain regions of Huntington disease patients, suggesting involvement in neurodegenerative processes. Overexpression induces apoptosis, which can be inhibited by ICE family-specific inhibitors. Multiple alternatively spliced variants exist, though not all are fully characterized.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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