Thermo Fisher Scientific HNMT Polyclonal Antibody
Thermo Fisher Scientific의 HNMT Polyclonal Antibody는 인간, 마우스, 랫트에 반응하는 토끼 유래 항체입니다. Western blot, IHC, ICC/IF에 적합하며, 친화 크로마토그래피로 정제된 액상 형태입니다. HNMT 단백질의 내인성 수준 검출에 사용됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human HNMT (Accession P50135), corresponding to amino acid residues L211–E261 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | −20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2852020 |
Product Specific Information
Antibody detects endogenous levels of total HNMT.
Target Information
Histamine in mammals is metabolized by two major pathways: N(tau)-methylation via histamine N-methyltransferase (HNMT) and oxidative deamination via diamine oxidase.
HNMT is a cytosolic enzyme that uses S-adenosyl-L-methionine as the methyl donor.
In the mammalian brain, histamine neurotransmitter activity is primarily regulated by N(tau)-methylation, since diamine oxidase is not present in the central nervous system.
A common genetic polymorphism can affect the enzyme activity levels of HNMT in red blood cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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