Thermo Fisher Scientific DKK3 Polyclonal Antibody
DKK3 단백질을 인식하는 Rabbit Polyclonal 항체로 Western blot, IHC, ICC 등 다양한 응용에 사용 가능. Human, Mouse, Rat 등 반응. Affinity chromatography로 정제된 Liquid 형태, -20°C 보관. 연구용 전용 제품.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human DKK3 (Accession Q9UBP4), corresponding to amino acid residues E320–I350 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2852023 |
Product Specific Information
Antibody detects endogenous levels of total DKK3.
Target Information
DKK3, like DKK1, DKK2, and DKK4, possesses an N-terminal signal peptide and two conserved cysteine-rich domains separated by a linker region containing 10 cysteine residues each.
The second cysteine region has a putative lipid-binding function facilitating WNT/DKK interactions at the plasma membrane.
The linker region contains 50–55 amino acids in DKK1, DKK2, and DKK4, but only 12 amino acids in DKK3.
All DKKs have several potential cleavage sites for furin-type proteases.
Northern blot analysis shows DKK3 is widely expressed, with highest expression in heart, brain, and spinal cord. In situ hybridization reveals highest expression in mouse brain, eye, and heart.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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