Thermo Fisher Scientific Pan Ras Recombinant Rabbit Monoclonal Antibody (JF10-11)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:2,000

-

Immunocytochemistry (ICC/IF)

1:100-1:500

-

Product Specifications

Species Reactivity

Human, Mouse, Rat, Zebrafish

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

JF10-11

Immunogen

Synthetic peptide within Human Ras aa 21-64 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Pan Ras,uniProtId:P01111-1,ncbiNodeId:9606,antigenRange:21-64,antigenLength:189,antigenImageFileName:MA5-32561_Pan_Ras_P01111-1_Rabbit.svg,antigenImageFileNamePDP:MA5-32561_Pan_Ras_P01111-1_Rabbit_PDP.jpeg,sortOrder:1},{targetFamily:Pan Ras,uniProtId:P01112-1,ncbiNodeId:9606,antigenRange:21-64,antigenLength:189,antigenImageFileName:MA5-32561_Pan_Ras_P01112-1_Rabbit.svg,antigenImageFileNamePDP:MA5-32561_Pan_Ras_P01112-1_Rabbit_PDP.jpeg,sortOrder:2},{targetFamily:Pan Ras,uniProtId:P01116-1,ncbiNodeId:9606,antigenRange:21-64,antigenLength:189,antigenImageFileName:MA5-32561_Pan_Ras_P01116-1_Rabbit.svg,antigenImageFileNamePDP:MA5-32561_Pan_Ras_P01116-1_Rabbit_PDP.jpeg,sortOrder:3}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

TBS, pH 7.4, with 40% Glycerol, 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2809838

Product Specific Information

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

This gene belongs to the Ras oncogene family, whose members are related to the transforming genes of mammalian sarcoma retroviruses. The products encoded by these genes function in signal transduction pathways. These proteins can bind GTP and GDP, and they have intrinsic GTPase activity. This protein undergoes a continuous cycle of de- and re-palmitoylation, which regulates its rapid exchange between the plasma membrane and the Golgi apparatus. Mutations in this gene cause Costello syndrome, a disease characterized by increased growth at the prenatal stage, growth deficiency at the postnatal stage, predisposition to tumor formation, mental retardation, skin and musculoskeletal abnormalities, distinctive facial appearance and cardiovascular abnormalities. Defects in this gene are implicated in a variety of cancers, including bladder cancer, follicular thyroid cancer, and oral squamous cell carcinoma. Multiple transcript variants, which encode different isoforms, have been identified for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.