Thermo Fisher Scientific Complement C3 Recombinant Rabbit Monoclonal Antibody (JF10-30)
Recombinant rabbit monoclonal antibody recognizing human Complement C3. Validated for WB, ICC/IF, and Flow Cytometry. Offers high specificity, sensitivity, and lot-to-lot consistency. Supplied as liquid, 1 mg/mL in TBS with glycerol and BSA. For resear...
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:100 |
| Flow Cytometry (Flow) | 1:50–1:100 |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JF10-30 |
| Immunogen | Synthetic peptide within Human C3 (aa 1210–1248) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4 °C short term; for long-term storage, store at −20 °C, avoiding freeze/thaw cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2809843 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select optimal candidates for production.
Advantages include:
- High specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to the large rabbit immune repertoire
Target Information
Complement component C3 is a key protein in the complement system where classical and alternative activation pathways converge at the C3 activation step. Upon activation, C3 is cleaved into C3a and C3b.
C3 has a molecular mass of approximately 185 kDa and consists of two chains (110 kDa and 75 kDa) linked by disulfide bonds.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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