Thermo Fisher Scientific CD19 Recombinant Rabbit Monoclonal Antibody (JF100-06)
CD19 단백질을 인식하는 토끼 유래 재조합 단클론 항체로, Western blot, IHC, ICC, Flow cytometry에 적합합니다. 높은 특이성과 일관된 로트 간 재현성을 제공하며, 단기 4°C 및 장기 -20°C 보관이 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000–1:2,000 | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:1,000 | 1 publication |
| Immunocytochemistry (ICC/IF) | 1:50 | - |
| Flow Cytometry (Flow) | 1:1,000 | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Mouse |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JF100-06 |
| Immunogen | Recombinant protein within Human CD19 (aa 294–556) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C. Avoid freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809837 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. Specific antibody DNA sequences from immunoreactive rabbits are cloned and screened to select optimal production candidates.
Advantages:
- High specificity and sensitivity
- Lot-to-lot consistency
- Animal origin–free formulation
- Broad immunoreactivity to diverse targets due to the large rabbit immune repertoire
Target Information
CD19 is a member of the immunoglobulin superfamily with two Ig-like domains. It is expressed on 100% of peripheral B cells and on certain myeloid leukemia cells.
CD19 serves as a key regulator of B cell proliferation and differentiation, acting as a co-receptor with the B cell receptor (BCR) to lower the activation threshold for antigen-dependent stimulation.
Mutations in CD19 can cause hypogammaglobulinemia, while overexpression leads to B cell hyperactivity.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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