Thermo Fisher Scientific MMP3 Polyclonal Antibody, Biotin, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

Amino Acid Sequence of Recombinant Protein:
MRTFPGIPKW RKTHLTYRIV NYTPDLPKDA VDSAVEKALK VWEEVTPLTF SRLYEGEADI MISFAVREHG DFYPFDGPGN VLAHAYAPGP GINGDAHFDD DEQWTKDTTG TNLFLVAAHE IGHSLGLFHS ANTEALMYPL YHSLTDLTRF RLSQDDINGI QSLYGPPPDS PETPLVPTEP VPPEPGTPAN CDPALSFDAV STLRGEILIF KDRHFWRKSL RKLEPELHLI SSFWPSLPSG VDAAYEVTSK DLVFIFKGNQ FWAIRGNEVR AGYPRGIHTL GFPPTVRKID AAISDKEKNK TYFFVEDKYW RFDEKRNSME PGFPKQIAED FPGIDSKIDA VFEEFGFFYF FTGSSQLEFD PNAKKVTHTL KSNSWLNC

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human MMP-3. Anti-Human MMP-3-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect Human MMP-3 by sandwich ELISA (100 µL/well), use 0.25–1.0 µg/mL of this antibody. In conjunction with PeproTech Polyclonal Anti-Human MMP-3 (500-P324) as a capture antibody, detection sensitivity is 2000–4000 pg/mL of Recombinant Human MMP-3.

Western Blot:
To detect Human MMP-3 by Western Blot, use 0.1–0.2 µg/mL. Detection limit for Recombinant Human MMP-3 is 1.5–3.0 ng/lane under reducing or non-reducing conditions.

Package Information:
500-P324BT-1MG is provided as 2 × 500 µg.

Target Information

MMP3 (stromelysin-1) belongs to the peptidase M10A subfamily. It is secreted in its pro-protein form and activated by extracellular proteinase cleavage. MMP3 binds to zinc and calcium ions and exhibits metallo-endopeptidase activity. The active enzyme degrades collagen, gelatin, fibronectin, and laminin, and is involved in wound repair and tissue remodeling. Mutations in the gene can result in coronary heart disease 6.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.