Thermo Fisher Scientific Mouse anti-Rat IgG2a Secondary Antibody, FITC, eBioscience

Thermo Fisher Scientific Mouse anti-Rat IgG2a Secondary Antibody, FITC, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• eFluor 570
• eFluor 615
• eFluor 660
• PE
• PerCP-eFluor 710
• Custom conjugation available

Product Specific Information

The monoclonal antibody r2a-21B2 recognizes Rat IgG2a antibodies and can be used as a secondary reagent in flow cytometry and microscopy.
It does not cross-react with other rat isotypes or mouse immunoglobulins.

Applications Reported:

• Flow cytometric analysis
• Immunohistochemical staining (paraffin-embedded tissue)
• Immunocytochemistry

Applications Tested:
Tested by flow cytometry and immunohistochemistry using rat IgG2a primary antibody.
Recommended usage: ≤0.5 µg/test for Flow Cytometry or 10 µg/mL for IHC.
A test is defined as antibody amount staining a cell sample in 100 µL final volume.
Cell number range: 10⁵–10⁸ cells/test.
Titrate for optimal performance.

Excitation/Emission: 488 nm / 520 nm
Laser: Blue Laser

Target Information

Anti-Rat secondary antibodies are affinity-purified with defined specificity for rat immunoglobulins.
They are useful for detection, sorting, or purification of target antibodies and enable multiple detection systems (HRP, AP, fluorescence).
Secondary antibodies enhance sensitivity through signal amplification and are typically generated by immunizing host animals with pooled rat immunoglobulins, followed by purification and modification (affinity chromatography, fragmentation, label conjugation).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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