Thermo Fisher Scientific Mouse IgG2b kappa Isotype Control (eBMG2b), FITC, eBioscience
FITC로 결합된 Mouse IgG2b kappa 동형 대조 항체로, Flow Cytometry, IHC, ICC/IF 등 다양한 면역분석에 사용 가능. Blue Laser(488 nm)에서 최적 발광. 친화 크로마토그래피 정제, PBS/BSA 완충액 포함. 연구용으로만 사용.
- 카탈로그번호
- 11-4732-4x (2개 옵션)
- 판매단위
- pk
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Thermo Fisher Scientific Mouse IgG2b kappa Isotype Control (eBMG2b), FITC
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (IHC) | Assay-Dependent |
| Immunocytochemistry (ICC/IF) | Assay-Dependent |
| Flow Cytometry (Flow) | 5 µL (1 µg)/test |
| Control (Ctrl) | Assay-Dependent |
Product Specifications
| Specification | Description |
|---|---|
| Host/Isotype | Mouse / IgG2b, kappa |
| Class | Monoclonal |
| Type | Isotype Control |
| Clone | eBMG2b |
| Conjugate | FITC |
| Excitation/Emission Max | 498/517 nm |
| Form | Liquid |
| Concentration | 5 µL/test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10598655 |
Available Formats
- Unconjugated
- Alexa Fluor 488
- Alexa Fluor 532
- Alexa Fluor 647
- Alexa Fluor 660
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 395, 496, 563, 615, 661, 737, 805
- Brilliant Violet 421, 480, 605, 650, 711, 786
- eFluor 450, 506, 570, 615, 660
- Functional Grade
- PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610
- PerCP-Cyanine5.5, PerCP-eFluor 710
- Super Bright 436, 600, 645, 702, 780
- Request custom conjugation available
Product Specific Information
- Description: Monoclonal mouse IgG2b, kappa is useful as an isotype control immunoglobulin.
- Applications Reported: Suitable for immunocytochemistry, immunohistochemistry, and flow cytometric analysis.
- Applications Tested: Pre-titrated and tested for intracellular flow cytometric analysis at 5 µL (1 µg)/test per 100 µL final volume.
- Recommended cell number: 10⁵–10⁸ cells/test (empirically determined).
- Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.
- Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Mouse IgG2b is a subtype of immunoglobulin G produced by B cells in response to antigens. It is the third most abundant IgG subtype in serum and plays a key role in adaptive immune responses, including opsonization, complement activation, and antibody-dependent cell-mediated cytotoxicity.
It is also involved in regulating immune responses such as T cell activation and B cell inhibition.
Mouse IgG2b is widely used in research and diagnostics (ELISA, Western blot, IHC) and in therapeutic development (immunotherapy, vaccine research).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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