Thermo Fisher Scientific F4/80 Monoclonal Antibody (BM8), FITC, eBioscience

Applications and Tested Dilution

Product Specifications

Additional Available Formats

Unconjugated, Alexa Fluor 488, Alexa Fluor 532, Alexa Fluor 561, Alexa Fluor 660, Alexa Fluor 700, APC, APC-eFluor 780, Biotin, Brilliant UV 395, Brilliant UV 563, Brilliant UV 661, Brilliant UV 805, Brilliant Violet 421, Brilliant Violet 650, Brilliant Violet 711, eFluor 450, eFluor 506, eFluor 570, eFluor 660, Functional Grade, PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610, PerCP-Cyanine5.5, Super Bright 436, Super Bright 600, Super Bright 645, Super Bright 702, Super Bright 780, Custom conjugation available.

Product Specific Information

The BM8 monoclonal antibody reacts with mouse F4/80 antigen (~160 kDa surface receptor) belonging to the EGF-TM7 family. It contains seven EGF-like extracellular domains, seven transmembrane regions, and an intracellular C-terminal domain homologous to other TM7 superfamily members.
F4/80 is expressed by most mature macrophages and serves as a key marker for this cell population. It can also be expressed on peritoneal eosinophils, Langerhans cells, and certain dendritic cells. Expression begins early in myeloid development and can be upregulated with M-CSF stimulation. Some macrophage subsets, particularly in lymphoid tissues, may lack F4/80 expression.

Applications Reported: Flow cytometric analysis.
Applications Tested: Tested on mouse resident peritoneal exudate cells at ≤0.5 µg/test (100 µL final volume, 10⁵–10⁸ cells/test). Titrate for optimal performance.
Excitation: 488 nm
Emission: 520 nm
Laser: Blue Laser
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

F4/80 antigen is a 160 kDa cell surface glycoprotein, part of the EGF-TM7 family, sharing 68% amino acid identity with human EMR1. Expression varies by macrophage maturation and activation state. It is found on macrophages in multiple tissues (Kupffer cells, Langerhans, microglia, gut lamina propria, peritoneal cavity, lung, thymus, bone marrow stroma, spleen red pulp) and some dendritic cells, but absent in T-cell areas of spleen and lymph nodes.
Although ligands and functions are not fully defined, studies suggest a role in efferent CD8⁺ regulatory T cell generation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.