Thermo Fisher Scientific Ankyrin 1 Monoclonal Antibody (N388A/10), PerCP
Ankyrin 1 단백질 검출용 PerCP-conjugated 단클론 항체로, Western blot, IHC, ICC/IF에 사용 가능. 인간, 마우스, 랫트 반응성. 1 mg/mL 농도, Protein G 정제, 보존제 없음. Ankyrin-R 특이적이며 Ankyrin-B/G와 교차반응 없음.
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Applications and Tested Dilution
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1:1,000 | |
| Immunohistochemistry (IHC) | Assay-dependent | |
| Immunocytochemistry (ICC/IF) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | N388A/10 |
| Immunogen | Fusion protein amino acids 1–1881 (full-length) of human Ankyrin-R |
| Conjugate | PerCP |
| Excitation/Emission Max | 482 / 675 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.4, with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2932185 |
Additional Formats
Product Specific Information
- A 1:100 dilution of MA5-45731 detects Ankyrin R in 20 µg of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
- Detects approximately 200 kDa.
- Does not cross-react with Ankyrin-B or Ankyrin-G.
- This antibody was formerly sold as clone S388A-10.
Target Information
Ankyrins are a family of proteins that link integral membrane proteins to the spectrin-actin cytoskeleton and play key roles in cell motility, activation, proliferation, and maintenance of specialized membrane domains. Multiple isoforms of ankyrin are expressed in a tissue-specific and developmentally regulated manner.
Ankyrin 1, the prototype of this family, was first discovered in erythrocytes and later found in brain and muscle. Mutations in erythrocytic ankyrin 1 are associated with hereditary spherocytosis. Alternative splicing in the regulatory domain produces different isoforms, including truncated muscle-specific forms from alternate promoters.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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