Thermo Fisher Scientific Ankyrin 1 Monoclonal Antibody (N388A/10), FITC
Ankyrin 1 단백질을 인식하는 FITC 결합 단일클론 항체로, Western blot, IHC, ICC 등 다양한 응용에 적합. Human, Mouse, Rat 반응성. 단백질 G 정제 및 보존제 무첨가. 4°C 암소 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (IHC) | Assay-dependent |
| Immunocytochemistry (ICC/IF) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | N388A/10 |
| Immunogen | Fusion protein amino acids 1–1881 (full-length) of human Ankyrin-R |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 9.1 mM sodium bicarbonate/PBS, pH 7.4, with 640.91 mM DMSO, 136.36 mM ethanolamine |
| Contains | No preservative |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2932184 |
Additional Formats
- Unconjugated (MA5-27723)
- APC (MA5-45729)
- PE (MA5-45732)
- PerCP (MA5-45731)
- Custom conjugation available on request
Product Specific Information
A 1:100 dilution of MA5-45730 was sufficient for detection of Ankyrin R in 20 µg of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. Detects approximately 200 kDa. Does not cross-react with Ankyrin-B or Ankyrin-G.
This antibody was formerly sold as clone S388A-10.
Target Information
Ankyrins are a family of proteins that link integral membrane proteins to the underlying spectrin-actin cytoskeleton and play key roles in cell motility, activation, proliferation, contact, and maintenance of specialized membrane domains. Multiple isoforms are expressed in a tissue-specific, developmentally regulated manner. Ankyrin 1, the prototype, was first discovered in erythrocytes and later found in brain and muscles. Mutations in erythrocytic ankyrin 1 are associated with hereditary spherocytosis. Complex alternative splicing in the regulatory domain produces different isoforms, including truncated muscle-specific variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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