Thermo Fisher Scientific BNIP3 Polyclonal Antibody, DyLight 650
BNIP3 단백질을 검출하기 위한 DyLight 650 결합 토끼 폴리클로날 항체. Western blot 및 면역세포염색에 적합하며, 651/673 nm 형광 특성을 가짐. 액상 형태로 4°C 암소 보관. 연구용으로만 사용 가능.
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Applications
Western Blot (WB)
- Tested Dilution: 1:500–1:1,000
Immunocytochemistry (ICC/IF)
- Tested Dilution: 1:50–1:200
Product Specifications
| 항목 | 내용 |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide against an N-terminal region (within residues 1–50) of the human BNIP3 protein |
| Conjugate | DyLight™ 650 |
| Excitation / Emission Max | 651 / 673 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Wet ice |
| RRID | AB_11153251 |
Product Specific Information
In Western blot, a band is observed in hypoxic samples at approximately 30 and 60 kDa, representing the BNIP3 protein.
Some non-specific bands may also appear.
It is recommended to run normoxic samples alongside hypoxic samples as a negative control.
Target Information
NIP3 is a member of the BCL2/adenovirus E1B 19 kDa-interacting protein (BNIP) family. It interacts with the E1B 19 kDa protein responsible for protection against virally induced cell death, as well as BCL2, another apoptotic protector.
NIP3 contains a BH3 domain and a transmembrane domain associated with pro-apoptotic function. This dimeric mitochondrial protein is known to induce apoptosis even in the presence of BCL2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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