Thermo Fisher Scientific BNIP3 Polyclonal Antibody, DyLight 650

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

-

Immunocytochemistry (ICC/IF)

1:50-1:200

-

Product Specifications

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide against an N-terminal region (within residues 1-50) of the human BNIP3 protein.

Conjugate

DyLight™ 650 DyLight™ 650 DyLight™ 650

Excitation/Emission Max

651/673 nm View spectra

Form

Liquid

Concentration

1 mg/mL

Storage conditions

4° C, store in dark

Shipping conditions

Wet ice

RRID

AB_11153251

Product Specific Information

In Western blot a band is seen in hypoxic samples at ~30 and 60 kDa, representing the BNIP3 protein.

There are some non-specific bands present as well.

It is recommended that normoxic samples are run next to the hypoxic samples as a negative control.

Target Information

NIP3 is a member of the BCL2/adenovirus E1B 19 kd-interacting protein (BNIP) family. It interacts with the E1B 19 kDa protein which is responsible for the protection of virally-induced cell death, as well as E1B 19 kDa-like sequences of BCL2, also an apoptotic protector. NIP3 contains a BH3 domain and a transmembrane domain, which have been associated with pro-apoptotic function. The dimeric mitochondrial protein is known to induce apoptosis, even in the presence of BCL2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.