Thermo Fisher Scientific BNIP3 Polyclonal Antibody, DyLight 488
BNIP3 단백질 검출용 DyLight 488 형광 접합 폴리클로날 항체. Western blot과 면역형광(ICC/IF)에 적합. 492/519 nm 형광 특성, 4°C 암소 보관. 인간 BNIP3 N-말단 펩타이드 기반 면역원 사용.
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Applications
Western Blot (WB)
- Tested Dilution: 1:500–1:1,000
Immunocytochemistry (ICC/IF)
- Tested Dilution: 1:50–1:200
Product Specifications
| 항목 | 내용 |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide against an N-terminal region (within residues 1–50) of the human BNIP3 protein |
| Conjugate | DyLight™ 488 |
| Excitation / Emission Max | 492 / 519 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Wet ice |
| RRID | AB_11152170 |
Product Specific Information
In Western blot, a band is seen in hypoxic samples at approximately 30 and 60 kDa, representing the BNIP3 protein.
There are some non-specific bands present as well.
It is recommended that normoxic samples be run next to the hypoxic samples as a negative control.
Target Information
NIP3 is a member of the BCL2/adenovirus E1B 19 kDa-interacting protein (BNIP) family.
It interacts with the E1B 19 kDa protein responsible for protection against virally induced cell death, as well as E1B 19 kDa-like sequences of BCL2, an apoptotic protector.
NIP3 contains a BH3 domain and a transmembrane domain, both associated with pro-apoptotic function.
This dimeric mitochondrial protein induces apoptosis even in the presence of BCL2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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