Thermo Fisher Scientific ATG5 Polyclonal Antibody, DyLight 488
ATG5 단백질을 인식하는 DyLight 488 형광 표지 폴리클로날 항체입니다. Western blot, IHC, ICC, Flow cytometry 등 다양한 응용에 사용 가능하며, 자가포식 관련 연구에 적합합니다. 항원 친화 정제 방식으로 제조되었으며 4°C 암조건에서 보관합니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1:500 | |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-Dependent | |
| Immunocytochemistry (ICC/IF) | Assay-Dependent | |
| Flow Cytometry (Flow) | Assay-Dependent | |
| ELISA | Assay-Dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bovine, Fruit fly, Fish, Guinea pig, Human, Mouse, Non-human primate, Pig, Reptile, Rat, Xenopus, Zebrafish |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide made to an N-terminal region (within residues 1–50) of the human ATG5 protein |
| Conjugate | DyLight™ 488 |
| Excitation/Emission Max | 492 / 519 nm |
| Form | Liquid |
| Concentration | 0.72 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 50 mM sodium borate |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11153930 |
Product Specific Information
- In Western Blot, a band is seen at ~56 kDa representing the ATG5–ATG12 complex. The molecular weight of human ATG5 is approximately 33 kDa.
- Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
- Suggested positive control: mouse wildtype ES cell lysate.
Target Information
ATG5 (Autophagy Related 5) is a key component of the autophagy pathway and plays an essential role in the apoptotic process. It is involved in various cellular processes including mitochondrial quality control, negative regulation of antiviral immune responses, lymphocyte development, MHC II antigen presentation, and adipocyte differentiation.
Following conjugation to ATG12, the ATG5–ATG12 complex participates in autophagosome formation and interacts with FADD to mediate autophagic cell death. It also associates with innate immune response proteins such as RIG-I and VISA, inhibiting type I interferon production and permitting viral replication.
Dysfunction of ATG5 has been linked to diseases such as spinocerebellar ataxia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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