Thermo Fisher Scientific APAF1 Polyclonal Antibody
APAF1 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot, ICC/IF, ELISA에 적합. Human, Mouse, Rat 시료 반응성. Affinity 정제, 액상 형태, -20°C 보관. 세포질 내 APAF1 검출에 유용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 470–600 of human APAF1 (NP_863651.1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.45 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.05% ProClin 300 |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2913626 |
Product Specific Information
- Positive test controls: Jurkat, 293T, Mouse testis
- Subcellular location: Cytoplasm
Immunogen sequence:
PHTLSPDQED CMYWYNFLAY HMASAKMHKE LCALMFSLDW IKAKTELVGP AHLIHEFVEY RHILDEKDCA VSENFQEFLS LNGHLLGRQP FPNIVQLGLC EPETSEVYQQ AKLQAKQEVD NGMLYLEWIN K
Target Information
The APAF1 gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several WD-40 domains, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, it forms an oligomeric apoptosome that activates caspase 9, triggering the caspase cascade leading to apoptosis. Alternative splicing results in multiple transcript variants encoding different isoforms.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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