Thermo Fisher Scientific Galectin 1 Polyclonal Antibody, Biotin, PeproTech

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-0.2 µg/mL

-

ELISA (ELISA)

0.25-1.0 µg/mL

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit

Class

Polyclonal

Type

Antibody

Immunogen

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View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Biotin Biotin Biotin

Form

Lyophilized

Concentration

0.1-1.0 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

Product Specific Information

AA Sequence of recombinant protein: ACGLVASNLN LKPGECLRVR GEVAPDAKSF VLNLGKDSNN LCLHFNPRFN AHGDANTIVC NSKDGGAWGT EQREAVFPFQ PGSVAEVCIT FDQANLTVKL PDGYEFKFPN RLNLEAINYM AADGDFKIKC VAFD.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human Galectin-1. Anti-Human Galectin-1-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA: To detect hGalectin-1 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human Galectin-1 (500-P210) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hGalectin-1.

Western Blot: To detect hGalectin-1 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hGalectin-1 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

500-P210BT-1MG will be provided as 2 x 500 µg

Target Information

Galectin-1 belongs to a large family of carbohydrate-binding proteins called lectins. Galectin-1can be either monomeric or homodimeric and is found in a wide variety of cells and tissue types. Galectin-1 can control cell growth, proliferation, induce apoptosis of activated T cells while it can also modulate cytokine secretion or inhibit pro-inflammatory cytokine production. Galectin-1 plays an important role in acute and chronic inflammation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.